<?xml version="1.0" encoding="UTF-8"?>
<NasReports xmlns="http://gensc.org/ns/gcdml/2.0"
    xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:gml="http://www.opengis.net/gml">
    <MIGSReports>
        <virus gcatID="not assigned" sourceName="not assigned" sourceVersion="1">
            <ncbiOrganismName>Roseobacter phage SIO1</ncbiOrganismName>
            <ncbiTaxId>136084</ncbiTaxId>
            <genomeProjectID>14308</genomeProjectID>
            <studyData>
                <projectName>Roseobacter phage SIO1</projectName>
                <submitToINSDC>true</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <instance>1989-10-30</instance>
                    </samplingTime>
                    <location>
                        <name>Scripps Pier, La Jolla, California, USA</name>
                        <lat>missing</lat><!-- manually determined -->
                        <lon>missing</lon><!-- manually determined -->
                    </location>
                    <amount>
                        <na/>
                    </amount>

                    <habitat>
                        <aquatic habitatDesc="marine">
                            <waterBody>
                                <depth>
                                    <na/>
                                </depth>
                            </waterBody>
                        </aquatic>
                    </habitat>
                </physicalMaterial>
            </originalSample>

            <isolate>
                <isolationConditions>
                    <na/>
                    <!-- no pid/doi: Rohwer, et al. 2002, The Complete Genomic Sequence of the Marine Phage Roseophage SIO1 Shares Homology with Nonmarine Phages -->
                </isolationConditions>
                <cultureCollection>
                    <na>not deposited</na>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <numReplicons>1</numReplicons>
                <originalHost>
                    <ncbiTaxId>2433</ncbiTaxId>
                    <!-- taxid=closest; hostname=Roseobacter phage SIO1 -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>inapplicable</healthDiseaseStatus>
                </originalHost>
                <pathogenicity>bacteria</pathogenicity>
                <propagation>lytic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>placeholder</method>
            </nucExtract>
            <!-- Tris.HCl/EDTA, formamide -->
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <sequencingMethod>dideoxysequencing</sequencingMethod>
                <assembly xmlns:g="http://gensc.org/gcdml">
                    <assemblyMethod>Sequencher</assemblyMethod>
                    <estimatedErrorRate>unknown</estimatedErrorRate>
                    <calculationMethod>unknown</calculationMethod>
                </assembly>
                <!-- Sequencher -->
                <sop/>
                <link/>
            </sequencing>
        </virus>

    </MIGSReports>

    <GCDReports>
        <virus gcatID="not assigned" sourceName="www.megx.net/user/mduhaime" sourceVersion="1">
            <ncbiOrganismName>Roseobacter phage SIO1</ncbiOrganismName>
            <ncbiTaxId>136084</ncbiTaxId>
            <genomeProjectID>14308</genomeProjectID>
            <studyData>
                <projectName>Roseobacter phage SIO1</projectName>
                <submitToINSDC>true</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <instance>1989-10-30</instance>
                    </samplingTime>
                    <location>
                        <name>Scripps Pier, La Jolla, California, USA</name>
                        <lat>32.88</lat>
                        <lon>-117.26</lon>
                        <extension>
                            <samplePointLocation>
                                <pos2D>32.88 -117.26</pos2D>
                                <determinationMethod>manual calculation based on sample location
                                    discription</determinationMethod>
                            </samplePointLocation>
                        </extension>
                    </location>
                    <amount>
                        <na/>
                    </amount>
                    <habitat>
                        <aquatic>
                            <waterBody>
                                <depth>
                                    <na/>
                                </depth>
                            </waterBody>
                        </aquatic>
                    </habitat>
                    <materialType>placeholder</materialType>
                </physicalMaterial>
            </originalSample>
            <isolate>
                <isolationConditions>
                    <na/>
                    <!-- no pid/doi: Rohwer, et al. 2002, The Complete Genomic Sequence of the Marine Phage Roseophage SIO1 Shares Homology with Nonmarine Phages -->
                </isolationConditions>
                <cultureCollection>
                    <na>not deposited</na>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <originalHost>
                    <ncbiTaxId>2433</ncbiTaxId>
                    <!-- Roseobacter phage SIO1 -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>inapplicable</healthDiseaseStatus>
                </originalHost>
                <pathogenicity>bacteria</pathogenicity>
                <propagation>lytic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>placeholder</method>
                <!-- Tris.HCl/EDTA, formamide -->
                <litReference>
                    <na/>
                    <!-- no pid/doi: Rohwer, et al. 2002, The Complete Genomic Sequence of the Marine Phage Roseophage SIO1 Shares Homology with Nonmarine Phages -->
                </litReference>
            </nucExtract>
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <complete sequencingMethods="dideoxysequencing">
                    <segment label="" size="39906" gc="46" sequencingMethods="dideoxysequencing">
                        <accession>AF189021</accession>
                        <assembly>
                            <assemblyMethod>Sequencher</assemblyMethod>
                            <estimatedErrorRate>unknown</estimatedErrorRate>
                            <calculationMethod>unknown</calculationMethod>
                        </assembly>
                    </segment>
                </complete>
            </sequencing>
        </virus>
    </GCDReports>

</NasReports>
