<?xml version="1.0" encoding="UTF-8"?>
<NasReports xmlns="http://gensc.org/ns/gcdml/2.0"
    xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:gml="http://www.opengis.net/gml"
    xsi:schemaLocation="http://gensc.org/gcdml ../../../base/gcdml.xsd">
    <MIGSReports>
        <virus gcatID="not assigned" sourceName="not assigned" sourceVersion="1">
            <ncbiOrganismName>Pseudoalteromonas phage PM2</ncbiOrganismName>
            <ncbiTaxId>10661</ncbiTaxId>
            <genomeProjectID xmlns:g="http://gensc.org/gcdml">14237</genomeProjectID>
            <studyData>
                <projectName>Pseudoalteromonas phage PM2</projectName>
                <submitToINSDC>true</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <interval>
                            <start>1960</start>
                            <stop>1969</stop>
                        </interval>
                    </samplingTime>
                    <location>
                        <name>Vina del Mar, Chile</name>
                        <lat>missing</lat>
                        <lon>missing</lon>
                        <!-- manually determined coords -->
                    </location>
                    <amount>
                        <na/>
                    </amount>

                    <habitat>
                        <aquatic habitatDesc="marine">
                            <waterBody>
                                <depth>
                                    <na/>
                                </depth>
                            </waterBody>
                        </aquatic>
                    </habitat>
                </physicalMaterial>
            </originalSample>

            <isolate>
                <isolationConditions>
                    <doi>10.1016/0042-6822(68)90094-9</doi>
                </isolationConditions>
                <cultureCollection>
                    <identifier>ATCC 27025-B1</identifier>
                </cultureCollection>
                <cultureCollection>
                    <identifier>HER 254</identifier>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <numReplicons>1</numReplicons>
                <originalHost>
                    <ncbiTaxId>28107</ncbiTaxId>
                    <!-- Pseudoalteromonas espejiana BAL-31; culture colleciton: HER 1254 -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>inapplicable</healthDiseaseStatus>
                </originalHost>
                <pathogenicity>bacteria</pathogenicity>
                <propagation>lytic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>Wheatcroft and Williams</method>
            </nucExtract>
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <!-- primer walking -->
                <sequencingMethod>dideoxysequencing</sequencingMethod>
                <assembly xmlns:g="http://gensc.org/gcdml">
                    <assemblyMethod>XGAP</assemblyMethod>
                    <estimatedErrorRate>unknown</estimatedErrorRate>
                    <calculationMethod>unknown</calculationMethod>
                </assembly>
                <sop/>
                <link/>
            </sequencing>
        </virus>

    </MIGSReports>

    <GCDReports>
        <virus gcatID="not assigned" sourceName="www.megx.net/user/mduhaime" sourceVersion="1">
            <ncbiOrganismName/>
            <ncbiTaxId>10661</ncbiTaxId>
            <genomeProjectID xmlns:g="http://gensc.org/gcdml">not assigned</genomeProjectID>
            <studyData>
                <projectName>Pseudoalteromonas phage PM2</projectName>
                <submitToINSDC>true</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <interval>
                            <start>1960</start>
                            <stop>1969</stop>
                        </interval>
                    </samplingTime>
                    <location>
                        <name/>
                        <lat>-33.07</lat>
                        <lon>-71.68</lon>
                        <!-- manually determined coords -->
                        <extension>
                            <samplePointLocation>
                                <pos2D>-33.07 -71.68</pos2D>
                                <determinationMethod>manual calculation based on sample location
                                    discription</determinationMethod>
                            </samplePointLocation>
                        </extension>
                    </location>
                    <amount>
                        <na/>
                    </amount>
                    <habitat>
                        <aquatic>
                            <waterBody>
                                <depth>
                                    <na/>
                                </depth>
                            </waterBody>
                        </aquatic>
                    </habitat>
                    <materialType>placeholder</materialType>
                </physicalMaterial>
            </originalSample>
            <isolate>
                <isolationConditions>
                    <doi>10.1006/viro.1999.9838</doi>
                </isolationConditions>
                <cultureCollection>
                    <identifier>ATCC 27025-B1</identifier>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <originalHost>
                    <ncbiTaxId>28107</ncbiTaxId>
                    <!-- Pseudoalteromonas espejiana BAL-31, host in culture collection: ATCC 27025 -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>inapplicable</healthDiseaseStatus>
                </originalHost>
                <alternateHost>
                    <ncbiTaxId>96059</ncbiTaxId>
                    <!-- Pseudoalteromonas sp. ER72M2, this was the host used for sequencing, strain was provided by Dr. Leonard Mindich (Public Health Research Institute, New York) -->
                    <hostClass>laboratory host</hostClass>
                    <healthDiseaseStatus>inapplicable</healthDiseaseStatus>
                </alternateHost>
                <pathogenicity>bacteria</pathogenicity>
                <propagation>lytic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>Wheatcroft and Williams</method>
                <litReference>
                    <doi>10.1006/viro.1999.9837</doi>
                </litReference>
            </nucExtract>
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <!-- primer walking -->
                <sop/>
                <link/>
                <complete sequencingMethods="dideoxysequencing">
                    <segment label="" size="10079" gc="42" sequencingMethods="dideoxysequencing">
                        <accession>AF155037</accession>
                        <assembly>
                            <assemblyMethod>XGAP</assemblyMethod>
                            <estimatedErrorRate>unknown</estimatedErrorRate>
                            <calculationMethod>unknown</calculationMethod>
                        </assembly>
                    </segment>
                </complete>
            </sequencing>
        </virus>
    </GCDReports>

</NasReports>
