<?xml version="1.0" encoding="UTF-8"?>
<NasReports xmlns="http://gensc.org/ns/gcdml/2.0"
    xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:gml="http://www.opengis.net/gml">
    <MIGSReports>
        <virus gcatID="not assigned" sourceName="not assigned" sourceVersion="1">
            <ncbiOrganismName>Phage phiJL001</ncbiOrganismName>
            <ncbiTaxId>279383</ncbiTaxId>
            <genomeProjectID>16076</genomeProjectID>
            <studyData>
                <projectName>Phage phiJL001</projectName>
                <submitToINSDC>true</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <instance>1999-08-24</instance>
                    </samplingTime>
                    <location>
                        <name>Tennessee Reef, Key Largo, Florida, USA</name>
                        <lat>missing</lat>
                        <!-- manually determined coords -->
                        <lon>missing</lon>
                        <!-- manually determined coords -->
                    </location>
                    <amount>
                        <na/>
                    </amount>
                    <habitat>
                        <aquatic habitatDesc="marine">
                            <waterBody>
                                <depth>
                                    <measure values="10" uom="m"/>
                                </depth>
                            </waterBody>
                        </aquatic>
                    </habitat>
                </physicalMaterial>
            </originalSample>

            <isolate>
                <isolationConditions>
                    <doi>10.1128/AEM.71.3.1598-1609.2005</doi>
                </isolationConditions>
                <cultureCollection>
                    <na>not deposited</na>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <numReplicons>1</numReplicons>
                <originalHost>
                    <ncbiTaxId>278678</ncbiTaxId>
                    <!-- host name: alpha proteobacterium JL001; host is sponge associated, isolated from Ircinia strobilina at same date and location as virus -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>alive</healthDiseaseStatus>
                </originalHost>
                <pathogenicity>placeholder</pathogenicity>
                <propagation>lysogenic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>Phenol/Chloroform</method>
            </nucExtract>
            <!-- Sambrook -->
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <sequencingMethod>dideoxysequencing</sequencingMethod>
                <assembly xmlns:g="http://gensc.org/gcdml">
                    <assemblyMethod>Phrap</assemblyMethod>
                    <estimatedErrorRate>unknown</estimatedErrorRate>
                    <calculationMethod>unknown</calculationMethod>
                </assembly>
                <sop/>
                <link/>
            </sequencing>
        </virus>
    </MIGSReports>

    <GCDReports>
        <virus gcatID="not assigned" sourceName="www.megx.net/user/mduhaime" sourceVersion="1">
            <ncbiOrganismName>Phage phiJL001</ncbiOrganismName>
            <ncbiTaxId>279383</ncbiTaxId>
            <genomeProjectID>16076</genomeProjectID>
            <studyData>
                <projectName>Phage phiJL001</projectName>
                <submitToINSDC>false</submitToINSDC>
                <submitToSraOrEna>false</submitToSraOrEna>
                <submitToTraceArchives>false</submitToTraceArchives>
            </studyData>
            <originalSample>
                <physicalMaterial>
                    <samplingTime>
                        <instance>1999-08-24</instance>
                    </samplingTime>
                    <location>
                        <name>Tennessee Reef, Key Largo, Florida, USA</name>
                        <lat>24.75</lat>
                        <!-- manually determined coords -->
                        <lon>-80.79</lon>
                        <!-- manually determined coords -->
                        <extension>
                            <description>host is sponge associated, isolated from Ircinia strobilina
                                at same date and location as virus</description>
                            <samplePointLocation>
                                <pos2D>24.75 -80.79</pos2D>
                                <determinationMethod>manual calculation based on sample location
                                    discription</determinationMethod>
                            </samplePointLocation>
                        </extension>
                    </location>
                    <amount>
                        <measure xmlns:g="http://gensc.org/gcdml" uom="l" values="20"/>
                    </amount>
                    <habitat>
                        <aquatic>
                            <waterBody>
                                <depth>
                                    <measure xmlns:g="http://gensc.org/gcdml" uom="m" values="10"/>
                                </depth>
                                <temperature uom="C" values="29.09"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <salinity uom="PSU" values="36.08"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <nitrate uom="micromol/l" values="0.32"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <dissolvedOxygen uom="ml/l" values="4.48"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <phosphate uom="micromol/l" values="0"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <anyParameter name="silicate" uom="micromol/l" values="2.47"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <anyParameter name="oxygen saturation" uom="ml/l" values="103.33"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                                <anyParameter name="oxygen utilization" uom="ml/l" values="-0.14"
                                    comment="megx.net WOA05 data interpolation: August average, 10 m"/>
                            </waterBody>
                        </aquatic>
                    </habitat>
                    <materialType>placeholder</materialType>
                </physicalMaterial>
            </originalSample>
            <isolate>
                <isolationConditions>
                    <doi>10.1128/AEM.71.3.1598-1609.2005</doi>
                </isolationConditions>
                <cultureCollection>
                    <na>not deposited</na>
                </cultureCollection>
                <subspecificGeneticLineage>inapplicable</subspecificGeneticLineage>
                <originalHost>
                    <ncbiTaxId>278678</ncbiTaxId>
                    <!-- host name: alpha proteobacterium JL001; host is sponge associated, isolated from Ircinia strobilina at same date and location as virus -->
                    <hostClass>environmental source</hostClass>
                    <healthDiseaseStatus>alive</healthDiseaseStatus>
                </originalHost>
                <pathogenicity>bacteria</pathogenicity>
                <propagation>lysogenic</propagation>
                <encodedTraits/>
            </isolate>
            <nucExtract>
                <method>Phenol/Chloroform</method>
                <!-- Sambrook -->
                <litReference>
                    <doi>10.1128/AEM.71.3.1598-1609.2005</doi>
                </litReference>
            </nucExtract>
            <sequencing>
                <nucleicAcidAmplification>pcr</nucleicAcidAmplification>
                <complete sequencingMethods="dideoxysequencing">
                    <segment label="" size="63649" gc="62" sequencingMethods="dideoxysequencing">
                        <accession>AY576273</accession>
                        <assembly>
                            <assemblyMethod>Phrap</assemblyMethod>
                            <estimatedErrorRate>unknown</estimatedErrorRate>
                            <calculationMethod>unknown</calculationMethod>
                        </assembly>
                    </segment>
                </complete>
            </sequencing>
        </virus>
    </GCDReports>

</NasReports>

